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ناقل بیان یوکاریوتی
If a eukaryotic expression vector is to be used as a plasmid (i.e.
The major features of a eukaryotic expression vector are a eu karyotic transcription unit with a pro moter (p), a multiple cloning site (MCS) in which to insert a target gene, and a DNA segment with transcription termi nation and polyadenylation signals (t); a eukaryotic selectable marker (ESM) gene; an origin of replication that func tions in the eukaryotic host cell (orieuk); an origin of replication that functions in E. coli (oriE); and an E. coli selectable marker (e.g.
All mammalian expression vectors tend to have similar features and are not very different in design from other eukaryotic expression vectors.
All eukaryotic expression vectors have the same basic format.
To simplify both maintenance and recombi nant DNA manipulations, eukaryotic expression vectors are routinely maintained in E. coli.
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